| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bradyrhizobium diazoefficiens | CATGATCAAGCGCTGCACC | TCCTTCAGCGTGACGTAGC | 59.94 | 59.79 | 183 | 88 |
100.00%
|
100.00%
|
| Primer 2 | Bradyrhizobium diazoefficiens | CGCCAACGTCCACGAAATC | ATGCTCCGAATGCGTTGC | 59.87 | 59.21 | 178 | 87 |
100.00%
|
100.00%
|
| Primer 3 | Bradyrhizobium diazoefficiens | ATGTCGAGGTCGCGATCAC | CGCGAGTTTGAAGCCTGTG | 59.94 | 59.79 | 214 | 85 |
100.00%
|
100.00%
|
| Primer 4 | Bradyrhizobium diazoefficiens | AGGCTCAACGATCGTCTGC | TTTTCGCGTCGGCTTGTC | 60.15 | 59.07 | 177 | 82 |
100.00%
|
100.00%
|
| Primer 5 | Bradyrhizobium diazoefficiens | GTGCGTGAACTTCGTCTGC | TGTCGCCACCTCCAAATCC | 59.80 | 60.00 | 244 | 80 |
100.00%
|
100.00%
|
| Primer 6 | Bradyrhizobium diazoefficiens | TCGCGACGAGGACATTCTG | ACCGAGCCTAAGAACGTGC | 59.86 | 60.08 | 153 | 80 |
100.00%
|
100.00%
|
| Primer 7 | Bradyrhizobium diazoefficiens | ACCAGGCTCAACGATCGTC | TTTTCGCGTCGGCTTGTC | 59.78 | 59.07 | 180 | 78 |
100.00%
|
100.00%
|
| Primer 8 | Bradyrhizobium diazoefficiens | CTGAAACCTGGGTCGCCAT | TGGCGTTGGCGATGATGT | 60.00 | 60.05 | 225 | 76 |
100.00%
|
100.00%
|
| Primer 9 | Bradyrhizobium diazoefficiens | TGAAACCTGGGTCGCCATC | TGGCGTTGGCGATGATGT | 60.00 | 60.05 | 224 | 76 |
100.00%
|
100.00%
|
| Primer 10 | Bradyrhizobium diazoefficiens | TGAAACCTGGGTCGCCAT | TGGCGTTGGCGATGATGT | 58.83 | 60.05 | 224 | 76 |
100.00%
|
100.00%
|